کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2134305 | 1087461 | 2009 | 7 صفحه PDF | دانلود رایگان |
ObjectiveUsing a proteomic approach, we recently identified plasma CCL8 as a potential biomarker for diagnosis of graft-vs-host-disease (GVHD) in mice as well as humans. Because mass spectrometric analysis is only semi-quantitative, a quantitative method of measuring plasma CCL8 levels in mice is needed.Materials and MethodsWe established an enzyme-linked immunosorbent assay for the quantitative measurement of CCL8 concentrations in mouse plasma.ResultsOur newly established enzyme-linked immunosorbent assay revealed that the plasma CCL8 concentrations (mean ± standard error; n = 12) were 1287 ± 55.7 ng/mL and 1604 ± 110.8 ng/mL on days 7 and 14 after allogeneic bone marrow transplantation (BMT), respectively, while the plasma concentrations was 316.6 ± 16.3 ng/mL on day 7 after syngeneic BMT. A Western blotting analysis also showed a difference in the plasma CCL8 levels between the allogeneic and syngeneic BMT groups, as did clinical GVHD scores. Neither lipopolysaccharide nor poly(I:C) elevated the plasma CCL8 concentrations, although a dramatic increase in interleukin-6 was detected after both treatments.ConclusionAn elevated plasma CCL8 concentration may be a promising plasma marker for GVHD in mouse models.
Journal: - Volume 37, Issue 4, April 2009, Pages 525–531