Comparison of molecular markers expression in vacuum-assisted biopsies and surgical specimens of human breast carcinomas

In this study, we examined whether vacuum-assisted breast biopsy (VABB) specimens provide an accurate immunohistochemical assessment of estrogen receptors (ER), progesterone receptors (PR), c-erbB-2, and p53 proteins.Thirty-seven consecutive cases with a VABB diagnosis of ductal carcinoma in situ (DCIS) were included. The immunohistochemical expression of ER and PR, c-erbB-2, and p-53 was assessed in VABB and in surgical specimens with pathological concordance. The “clinically relevant concordance rate” (CRCR) was calculated.The CRCRs were 77.8%, 77.8%, and 89% for ER, PR, and c-erbB-2, respectively. Concerning both ER and PR, the clinically relevant expression was higher in VABB specimens. A difference in borderline significance arose concerning c-erbB-2 expression, pointing to more intense expression in the surgical specimens. The p53 percentage did not differ between the two groups.VABB specimens provide an accurate immunohistochemical assessment of molecular markers. However, bioptical-surgical discrepancies persisted even in this context of a larger quantity of tissue removed.