Characterization of local calcium signals in tubular networks of endoplasmic reticulum

To explain the large time and space scales of elementary calcium events in Xenopus oocytes it is assumed that the Ca2+ source is located on tubules of the endoplasmic reticulum, which provide local barriers for diffusion. The event duration, width and signal mass dependence on the total quantity of released Ca2+ is determined at different orientations of the scan line and different ionic currents. Excellent agreement with published data is obtained with on- and off-rate constants of the fluorescent indicator of 15 μM−1 s−1 and 2.55 s−1, respectively. It is found that one signal mass unit, calculated with the classical method that assumes spherical symmetry of the cytosolic space surrounding the release site, corresponds to 0.189 fC of released Ca2+ in the presence of a tubular network. It is estimated that release Ca2+ currents and amounts are randomly distributed, with averages of 0.165 pA and 3.66 fC per event and average release duration of 22.2 ms. The total quantity of liberated Ca2+ and the release current amplitude in the presence of endoplasmic reticulum tubules is predicted to be about one order of magnitude lower than estimated within the isotropic diffusion formalism. This could have implications in muscle cell Ca2+ imaging as well.