کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2167291 | 1092323 | 2011 | 5 صفحه PDF | دانلود رایگان |
CpG motifs have been advanced as agents that stimulate the maturation of DCs for immunotherapy. The present study tested the hypothesis that multiple doses of CpG-matured DC vaccine would be efficacious for complete eradication of experimentally-induced tumor. Accordingly, WEHI164 cells were implanted subcutaneously in the flank of BALB/c mice. During DC culture, tumor lysate was added to immature DCs followed by addition of CpG or non-CpG control 4–6 h later. A total of three doses of CpG or non-CpG control-matured DCs were injected around tumors. The results showed that multiple doses of CpG-matured DCs led to considerable decrease in cytotoxicity of lymphocytes and significantly increased tumor growth rate compared to a single dose. Further, mice which received three doses of the vaccine also displayed significant FoxP3 in tumor tissue. In conclusion, multiple doses of CpG-matured DCs exhibited decreased anti-tumor immunity in association with increased expression of FoxP3.
► Results indicate the opposite effect of CpG in dendritic cells maturation.
► Injection of multiple doses of CpG-matured DCs attenuates the efficacy of DC-based immunotherapy.
► Increase expression of FoxP3 in tumor was seen following CpG-matured DCs injections.
► Results could be considered in clinical application of CpG for immunotherapy of cancer.
Journal: Cellular Immunology - Volume 271, Issue 2, 2011, Pages 360–364