کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2167293 1092323 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative binding of soluble fragments (derCD23, sCD23, and exCD23) of recombinant human CD23 to CD21 (SCR 1-2) and native IgE, and their effect on IgE regulation
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Comparative binding of soluble fragments (derCD23, sCD23, and exCD23) of recombinant human CD23 to CD21 (SCR 1-2) and native IgE, and their effect on IgE regulation
چکیده انگلیسی

IgE, responsible for type I hypersensitivities, is regulated by interactions between its receptor, CD23, and co-receptor CD21. To examine comparative binding of recombinant human CD21 SCR 1-2 and native human IgE to CD23 plus the effect of CD23 on IgE production, we engineered recombinant soluble human CD23 fragments; (1) derCD23, (2) sCD23 and (3) exCD23, formed in vivo by proteolysis. SPR analysis revealed a progressive increment in affinity of soluble fragments for IgE, upon increasing length of CD23 “stalk” domain, exCD23 > sCD23 > derCD23. Soluble CD23 fragments and their oligomeric state are shown to fine-tune the immune response. Oligomers appear more important in enhancing IgE synthesis and monomers lacking the tail residues fail to bind CD21 yet bind membrane IgE and down-regulate IgE synthesis. Co-ligation of membrane IgE and CD21 through soluble CD23 monomers is disturbed. This study supports anti-allergic therapies involving stabilizing membrane CD23, or preventing shedding of soluble CD23.


► Production of recombinant soluble human CD23 fragments (1) derCD23, (2) sCD23 and (3) exCD23.
► HPLC analysis showed various oligomeric forms dependent on the length of “stalk” domain in protein.
► SPR analysis showed a progressive increment in affinity for IgE, on increasing length of CD23.
► Oligomers enhance IgE synthesis, while monomers bind membrane IgE and down-regulate synthesis.
► The immune response is dependent on soluble CD23 fragments and their oligomeric state.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 271, Issue 2, 2011, Pages 371–378
نویسندگان
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