کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2168125 1092377 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Capture of flowing human neutrophils by immobilised immunoglobulin: Roles of Fc-receptors CD16 and CD32
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
Capture of flowing human neutrophils by immobilised immunoglobulin: Roles of Fc-receptors CD16 and CD32
چکیده انگلیسی

We investigated capture and activation of flowing human neutrophils through their Fc-receptors, FcRγIIIB (CD16) and FcRγIIA (CD32). Immobilised platelets bearing murine monoclonal antibody against glycoprotein IIbIIIA were able to capture and activate flowing neutrophils. The activation response was inhibited by antibody blockade of neutrophil CD32. However, capture only occurred efficiently at wall shear stress below 0.1 Pa if platelet P-selectin was blocked. If neutrophils were perfused over immobilised human IgG, many adhered at 0.025 or 0.05 Pa, but not at 0.1 Pa. Adhesion was reduced by blockade of CD16 or CD32, but blockade of CD16 had the greater effect. When neutrophils were perfused over a combination of purified P-selectin and IgG, blockade of CD16 and CD32 inhibited activation of captured cells. Immunoglobulin deposited in tissue could capture and activate slow-flowing neutrophils. It might also potentiate inflammatory responses at higher stress if presented along with selectins. The dominant FcR for capture of neutrophils was CD16, but with murine antibody, CD32 played a greater role.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 241, Issue 1, May 2006, Pages 26–31
نویسندگان
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