Immunoassay of β2-microglobulin at oxide-coated heavily doped p-silicon electrodes

• Novel immunoassay of beta-2-microglobulin
• Immunoassay on the surface of oxide-coated heavily doped p-silicon
• Detection by hot electron-induced electrochemiluminescence at p-silicon electrodes

An immunoassay was carried out on disposable oxide-coated silicon chip electrodes made of heavily doped p-type silicon, simply thermally oxidized in room air in a laboratory oven. The present electrode chips allow injection of hot electrons into aqueous electrolyte solutions and can be utilized in detecting electrochemiluminescent labels in fully aqueous solutions. This is demonstrated with an immunoassay of β2-microglobulin using an aromatic Tb(III) chelate as an electrochemiluminescent label.