کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2184271 | 1095820 | 2015 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Structure of Zeste–DNA Complex Reveals a New Modality of DNA Recognition by Homeodomain-Like Proteins Structure of Zeste–DNA Complex Reveals a New Modality of DNA Recognition by Homeodomain-Like Proteins](/preview/png/2184271.png)
• Zeste binds DNA and plays key roles in transvection and Zeste–white interaction.
• The structure of the DNA binding domain of Zeste bound to DNA has been solved.
• ZesteDBD is a novel variant of the DNA binding motif of helix–turn–helix type.
• The unique structural elements of ZesteDBD determine its DNA binding mode.
• The structure expands the knowledge about homeodomain-like DNA binding proteins.
Drosophila Zeste is a DNA binding protein important for chromatin-targeted regulation of gene expression. It is best studied in the context of transvection—a mechanism of interallelic gene regulation involving paired chromosomes—and repression of the expression of white by Zeste mutants. Both of these functions depend on the DNA binding and self-association properties of Zeste, but the underlying structural basis remains unknown. Here we report the crystal structure of the DNA binding domain of Zeste in complex with a 19-bp DNA duplex containing the consensus recognition sequence motif. The structure reveals a helix–turn–helix Myb/homeodomain-like fold with the Zeste-specific insertion sequence forming a short helix and a long loop. Direct base contacts by the major groove binding helix principally account for the sequence-specific recognition, and backbone contacts via the Zeste-specific insertion are mainly responsible for the length requirement and the orientation of DNA. Our structural and biochemical characterizations of the DNA binding property of Zeste uncover an altered DNA binding modality of homeodomain-like proteins, and the structural information should facilitate the unraveling of the intricate mechanism of Zeste in regulation of gene expression.
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Journal: Journal of Molecular Biology - Volume 427, Issue 24, 4 December 2015, Pages 3824–3833