کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
222622 464283 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of moderate electric fields on inactivation kinetics of pectin methylesterase in tomatoes: The roles of electric field strength and temperature
ترجمه فارسی عنوان
اثر میدان های الکتریکی متوسط ​​بر روی سینتیک غیر فعال سازی متیل استراز پکتین در گوجه فرنگی: نقش قدرت و درجه حرارت الکتریکی
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی


• Moderate electric fields (MEF) activate PME near the optimal activity temperature.
• At higher temperatures, moderate electric fields accelerate inactivation of PME.
• The effect of PME activation and inactivation increases with electric field strength.
• Under MEF, PME appears to behave as if at a higher temperature than the bulk.

The objective of this study was to investigate the effect of Moderate Electric Fields (MEF) on pectin methylesterase (PME) activity in tomatoes. Inactivation kinetics parameters of MEF and conventional thermal treatments were determined for samples of identical prior source and pretreatment history, subjected to nearly identical linearly increasing temperature histories. The presence of electric field (60 Hz, sine wave) resulted in activation of PME at 70 (±1) °C, and accelerated inactivation at higher temperatures. This effect increased with field strength. The results may be interpreted in light of molecular motion calculations for PME, which suggest that enzyme molecules subjected to an oscillating electric field experience electrophoretic motion, and behave as if at a slightly higher temperature than that of the bulk, resulting in an activating effect at lower temperatures, but an inactivation effect at higher temperatures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Engineering - Volume 186, October 2016, Pages 17–26
نویسندگان
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