کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2402489 | 1102795 | 2013 | 7 صفحه PDF | دانلود رایگان |
The effect of vaccination on immune parameters of European sea bass, Dicentrarchus labrax, is not fully established, as well as surveyed throughout rearing till the commercial size. Furthermore, available information on the possible role of booster treatments is scarce.Sea bass juveniles were vaccinated against Listonella anguillarum using a commercial bivalent formulation administered by immersion (priming: 95 dph; booster: 165 dph) or by immersion (priming: 95 dph; booster: 165 dph) and subsequent i.p. injection (booster: 233 dph).Serum specific IgM and numbers of IgM+ cells in head kidney and spleen evidenced B-cell responses mainly after the immersion booster, accompanied by increased TcR-β transcripts and leucocyte respiratory burst.Immune enhancement was confirmed by the protection towards i.p. challenges with a virulent strain. RPS accounted for >70% in fish immersion-boosted and near 100% in fish further boosted i.p.Differently from usual farm practices, this innovative vaccination protocol proved to be highly effective. Booster treatments are therefore strongly recommended.
► An innovative vaccination protocol (priming + boosters) was assessed against Dicentrarchus labrax vibriosis.
► The immune response (aspecific and specific) and the protection towards challenge were measured.
► The booster treatments promoted aspecific and specific immune responses.
► The vaccination procedures achieved protection against the Listonella anguillarum challenge.
► The proposed vaccination protocol has proven to be highly effective.
Journal: Vaccine - Volume 31, Issue 8, 6 February 2013, Pages 1224–1230