کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2408739 | 1103188 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immune enhancing effects of recombinant bovine IL-18 on foot-and-mouth disease vaccination in mice model
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کلمات کلیدی
CFAFMDVHprtDTHPBMCLPART-PCRkDaBSA - BSAMTT - MTTReverse transcriptase PCR - PCR معکوس رونویسیbovine serum albumin - آلبومین سرم گاوsodium dodecyl sulfate-polyacrylamide gel electrophoresis - الکتروفورز ژل دوده سولفات سدیم پلی آکریل آمیدSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدP. pastoris - ای پاستورdelayed-type hypersensitivity - تاخیر نوع حساسیت بالاPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیLuria Bertani - لوریا برتانیhypoxanthine phosphoribosyl transferase - هیپوکسانتین فسفریبوسیل ترانسفرازMolecular weight - وزن مولکولیFoot and mouth disease virus - ویروس بیماری پا و دهانoptical density - چگالی نوریImmune adjuvant - کمپلکس ایمنیkilo-dalton - کیلو دالتون
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Foot-and-mouth disease (FMD) is a highly contagious disease in cloven-hoofed animals and can cause a considerable socio-economic loss for affected countries. Interleukin-18 (IL-18) is a pleiotropic cytokine and plays important role in both the development of a functional immune system as well as the response of the organism to infection. In the present study, bovine IL-18 (BoIL-18), Foot-and-mouth disease virus VP1 and VP1/BoIL-18 fusion genes were cloned and expressed in pichia pastoris (P. pastoris) and subsequently immune effects were evaluated to study the immune enhancing effects of recombinant BoIL-18 (rBoIL-18) on FMD vaccination. The results showed that the genes encoding for BoIL-18, VP1 and VP1/BoIL-18 are successfully expressed in P. pastoris and the expressed recombinant VP1 (rVP1) proteins could induce both humoral and marginal cell-mediated immune responses in mice, while the co-inoculation with rBoIL-18 could markedly enhance both of immune responses, and the inoculation of the fusion product rVP1/BoIL-18 showed even more dramatic immune responses, suggesting rBoIL-18 has a potential to enhance the efficacy of vaccination against FMDV infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vaccine - Volume 25, Issue 7, 26 January 2007, Pages 1257-1264
Journal: Vaccine - Volume 25, Issue 7, 26 January 2007, Pages 1257-1264
نویسندگان
Xi-Ju Shi, Bin Wang, Ming Wang,