کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2449369 1554075 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Proteomic investigation of protein profile changes and amino acid residue-level modification in cooked lamb longissimus thoracis et lumborum: The effect of roasting
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Proteomic investigation of protein profile changes and amino acid residue-level modification in cooked lamb longissimus thoracis et lumborum: The effect of roasting
چکیده انگلیسی


• Shotgun proteomic evaluation of protein modifications in dry heat-treated lamb
• Longer roasting reduced meat protein extractability in urea–thiourea solution
• Longer roasting also resulted in protein truncation.
• Some modifications that might influence nutritional properties, e.g., carboxyethyllysine, were observed after roasting.
• Actin and myoglobin may provide peptides as potential markers of cooking in lamb meat.

Protein modifications of meat cooked by typical dry-heat methods (e.g., roasting) are currently not well understood. The present study utilised a shotgun proteomic approach to examine the molecular-level effect of roasting on thin lamb longissimus thoracis et lumborum patties, in terms of changes to both the protein profile and amino acid residue side-chain modifications. Cooking caused aggregation of actin, myosin heavy chains and sarcoplasmic proteins. Longer roasting time resulted in significantly reduced protein extractability as well as protein truncation involving particularly a number of myofibrillar and sarcoplasmic proteins, e.g., 6-phosphofructokinase, beta-enolase, l-lactate dehydrogenase A chain, alpha-actinin-3, actin and possibly myosin heavy chains. Modifications that have potential influence on nutritional properties, including carboxyethyllysine and a potentially glucose-derived N-terminal Amadori compound, were observed in actin and myoglobin after roasting. This study provided new insights into molecular changes resulting from the dry-heat treatment of meat, such as commonly used in food preparation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Meat Science - Volume 119, September 2016, Pages 80–88
نویسندگان
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