کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2580235 1561607 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular and biochemical characterisation of human short-chain dehydrogenase/reductase member 3 (DHRS3)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Molecular and biochemical characterisation of human short-chain dehydrogenase/reductase member 3 (DHRS3)
چکیده انگلیسی


• DHRS3 is an integral membrane protein with its C-terminus in the cytosol.
• Androstenedione and estrone are substrates of DHRS3 in vitro.
• DHRS3 was inserted into proteoliposomes.
• Expression of DHRS3 protein was detected in the human liver, testes and small intestine.

Dehydrogenase/reductase (SDR family) member 3 (DHRS3), also known as retinal short-chain dehydrogenase/reductase (retSDR1) is a member of SDR16C family. This family is thought to be NADP(H) dependent and to have multiple substrates; however, to date, only all-trans-retinal has been identified as a DHRS3 substrate. The reductive reaction catalysed by DHRS3 seems to be physiological, and recent studies proved the importance of DHRS3 for maintaining suitable retinoic acid levels during embryonic development in vivo. Although it seems that DHRS3 is an important protein, knowledge of the protein and its properties is quite limited, with the majority of information being more than 15 years old.This study aimed to generate a more comprehensive characterisation of the DHRS3 protein. Recombinant enzyme was prepared and demonstrated to be a microsomal, integral-membrane protein with the C-terminus oriented towards the cytosol, consistent with its preference of NADPH as a cofactor. It was determined that DHRS3 also participates in the metabolism of other endogenous compounds, such as androstenedione, estrone, and DL-glyceraldehyde, and in the biotransformation of xenobiotics (e.g., NNK and acetohexamide) in addition to all-trans-retinal. Purified and reconstituted enzyme was prepared for the first time and will be used for further studies. Expression of DHRS3 was shown at the level of both mRNA and protein in the human liver, testis and small intestine. This new information could open other areas of DHRS3 protein research.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 234, 5 June 2015, Pages 178–187
نویسندگان
, , , , , , , ,