کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2598041 | 1132610 | 2006 | 8 صفحه PDF | دانلود رایگان |
To evaluate the genotoxic effects of lead (Pb) exposure, 25 workers in a workplace producing storage battery were monitored for three genetic end-points using micronucleus (MN) assay, comet assay and TCR gene mutation test. Twenty-five controls were matched with workers according to age, gender and smoking. The air Pb concentration in the workplace was 1.26 mg/m3. All subjects were measured for Pb concentration of blood by atom absorption spectrophotometry. The mean Pb concentration of blood in workers (0.32 mg/l) was significantly higher than that in controls (0.02 mg/l). The results of MN test showed that the mean micronuclei rate (MNR) and mean micronucleated cells rate (MCR) in workers were 9.04 ± 1.51‰ and 7.76 ± 1.23‰, respectively, which were significantly higher than those (2.36 ± 0.42‰ and 1.92 ± 0.31‰) in controls (P < 0.01). It was found in the comet assay that the mean tail length (MTL) of 25 workers and 25 controls were 2.42 ± 0.09 and 1.02 ± 0.08 μm, respectively, there was significant difference between workers and controls for MTL (P < 0.01), also the difference of the mean tail moment (MTM) between workers (0.85 ± 0.05) and controls (0.30 ± 0.09) was very significant (P < 0.01). However, in TCR gene mutation assay Mfs-TCR of workers and controls were 1.69 ± 0.15 × 10−4 and 1.74 ± 0.17 × 10−4, respectively, there was no significant difference between workers and controls (P > 0.05). The results of our study indicated that the genetic damage was detectable in 25 workers occupationally exposed to lead.
Journal: Toxicology - Volume 223, Issue 3, 15 June 2006, Pages 219–226