کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
26205 43940 2016 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electron transfer mediated decomposition of folic acid by photoexcited dimethoxophosphorus(V)porphyrin
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Electron transfer mediated decomposition of folic acid by photoexcited dimethoxophosphorus(V)porphyrin
چکیده انگلیسی


• We are aiming the construction of a fluorometry system to evaluate the biomolecule damaging-activity of photosensitizer using folic acid.
• Dimethoxophosphorus(V)tetraphenylporphyrin photosensitized folic acid decomposition.
• The initial step of this folic acid photodecomposition was explained by an electron transfer.
• This electron transfer proceeds as a diffusion-controlled reaction.
• Singlet oxygen-mediated decomposition of folic acid was also observed in deuterium oxide.

A water soluble porphyrin, dimethoxophosphorus(V)tetraphenylporphyrin (MP(V)TPP) photosensitized folic acid decomposition in aqueous solution, resulting in the formation of a strongly fluorescent pteridine compound. The quantum yield of folic acid decomposition by photoexcited MP(V)TPP could be determined by a flurometry. A possible mechanism of the initial process of this photodecomposition is direct oxidation through an electron transfer from folic acid to the excited singlet state of MP(V)TPP. It is considered that this electron transfer proceeds as a diffusion-controlled reaction. The quantum yields of the electron transfer and the following process of folic acid decomposition could be determined. In deuterium oxide, folic acid decomposition through the photosensitized singlet oxygen generation by MP(V)TPP was also observed. A fluorometry based on the formation of a strong fluorescent compound through the oxidized decomposition of folic acid may be applied to evaluate the biomolecule damaging-activity of photosensitizers. In addition, a protocol of this assay is proposed. This simple photosensitized reaction in aqueous solution may be used for a first screening of a photosensitizer for phototherapy.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology A: Chemistry - Volume 318, 1 March 2016, Pages 1–6
نویسندگان
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