کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2692903 1143483 2016 5 صفحه PDF ندارد دانلود کنید
عنوان انگلیسی مقاله
Determination of cytotoxicity of traditional Chinese medicine herbs, Rhizoma coptidis, Radix scutellariae, and Cortex phellodendri, by three methods
ترجمه فارسی عنوان
تعیین سمیت سلولی گیاهان سنتی طب چینی، coptidis ریزوم، scutellariae ریشه و Cortex phellodendri، با سه روش
کلمات کلیدی
coptidis ریزوم؛ scutellariae ریشه ؛ قشر phellodendri ؛ سلول های اپیتلیال قرنیه انسانی؛ سمیت سلولی؛ فلوسیتومتری
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی چشم پزشکی
چکیده انگلیسی

BackgroundMany herbs are used in traditional Chinese medicine TCM) for treatment of infections but their properties, in particular, their effects on normal cells have received little attention. This study investigated the cytotoxic properties of three TCM herbs with potential use in prevention and treatment of ocular infections, including Acanthamoeba keratitis.MethodThe study investigated cytotoxic effects of the herbal extracts of Rhizoma coptidis, Radix scutellariae, and Cortex phellodendri on human corneal epithelial cells using trypan blue staining, MTT production, and flow cytometry. Differences between herbs were determined using repeated measures one-way analysis of variance, followed by paired t-tests where appropriate.ResultsThese three herbs appeared to lack cytotoxicity when tested with trypan blue and MTT, but flow cytometry revealed that R. coptidis led to cell membrane damage.ConclusionLack of cytotoxicity of R. scutellariae and C. phellodendri extracts suggest that these are potentially suitable for use in ocular preparations. Only flow cytometry was able to accurately predict cytotoxic effects of extracts of TCM herbs on HCEC, demonstrating the importance of using a sensitive method of detection of cytotoxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Contact Lens and Anterior Eye - Volume 39, Issue 2, April 2016, Pages 128–132
نویسندگان
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