کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2792298 1568667 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Methionine restriction alters bone morphology and affects osteoblast differentiation
ترجمه فارسی عنوان
محدودیت متیونین باعث تغییر ساختار استخوان می شود و تمایز استئوآبلاست را تحت تاثیر قرار می دهد
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی علوم غدد
چکیده انگلیسی


• MR affected the growth rates of males and young females, but not aged females.
• CF and MR mice had similar intrinsic strength properties.
• Low methionine media attenuated bone differentiation genes in MC3T3-E1 preosteoblast cells.
• The lower bone mass in MR mice is appropriate for its smaller body size.

Methionine restriction (MR) extends the lifespan of a wide variety of species, including rodents, drosophila, nematodes, and yeasts. MR has also been demonstrated to affect the overall growth of mice and rats. The objective of this study was to evaluate the effect of MR on bone structure in young and aged male and female C57BL/6J mice. This study indicated that MR affected the growth rates of males and young females, but not aged females. MR reduced volumetric bone mass density (vBMD) and bone mineral content (BMC), while bone microarchitecture parameters were decreased in males and young females, but not in aged females compared to control-fed (CF) mice. However, when adjusted for bodyweight, the effect of MR in reducing vBMD, BMC and microarchitecture measurements was either attenuated or reversed suggesting that the smaller bones in MR mice is appropriate for its body size. In addition, CF and MR mice had similar intrinsic strength properties as measured by nanoindentation. Plasma biomarkers suggested that the low bone mass in MR mice could be due to increased collagen degradation, which may be influenced by leptin, IGF-1, adiponectin and FGF21 hormone levels. Mouse preosteoblast cell line cultured under low sulfur amino acid growth media attenuated gene expression levels of Col1al, Runx2, Bglap, Alpl and Spp1 suggesting delayed collagen formation and bone differentiation. Collectively, our studies revealed that MR altered bone morphology which could be mediated by delays in osteoblast differentiation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bone Reports - Volume 5, December 2016, Pages 33–42
نویسندگان
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