کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2798073 | 1155676 | 2008 | 7 صفحه PDF | دانلود رایگان |
ObjectivesThe objective of this work was to evaluate the effect of different glucose levels on the ATP, ADP and AMP hydrolysis in the platelets of diabetic, hypertensive and diabetic/hypertensive participants.MethodsThe activities of the enzymes NTPDase (ATP and ADP hydrolysis) and 5′-nucleotidase (AMP hydrolysis), and CD39 expression were analyzed in human blood platelets of diabetic (DM-2), hypertensive (HT) and diabetic/hypertensive (DM-2/HT) patients. To evaluate the interference of glucose and fructose in NTPDase and 5′-nucleotidase activities, experiments were performed with glucose, fructose and mannitol concentrations ranging from 5 to 30 mM in platelet-rich plasma (PRP). Pre-incubation times of 10, 120 min and 24 h were used.ResultsNTPDase and 5′-nucleotidase activities increased with increasing glucose and fructose concentrations (P < 0.001) and the different times of pre-incubation did not interfere in ectonucleotidases activities (P > 0.5). NTPDase and 5′-nucleotidase activities demonstrated a positive correlation between serum glucose levels and ATP and ADP hydrolysis in DM-2 and DM-2/HT patients. CD39 expression demonstrated that DM-2, HT and DM-2/HT groups presented a significant increase when compared to the control group (P < 0.004).ConclusionThe hydrolysis of adenine nucleotides is enhanced in platelets of patients with diabetes and hypertension. We observed that an increasing glucose concentration had a direct effect on ATP, ADP and AMP hydrolysis. Furthermore, CD39 expression was enhanced in all patients groups, indicating that these enzyme activities are related with diabetes and hypertension.
Journal: Diabetes Research and Clinical Practice - Volume 81, Issue 3, September 2008, Pages 351–357