کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2824191 1161628 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Construction of two vectors for gene expression in Trichoderma reesei
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی ژنتیک
پیش نمایش صفحه اول مقاله
Construction of two vectors for gene expression in Trichoderma reesei
چکیده انگلیسی

We report the construction of two filamentous fungi Trichoderma reesei expression vectors, pWEF31 and pWEF32. Both vectors possess the hygromycin phosphotransferase B gene expression cassette and the strong promoter and terminator of the cellobiohydrolase 1 gene (cbh1) from T. reesei. The two newly constructed vectors can be efficiently transformed into T. reesei with Agrobacterium-mediated transformation. The difference between pWEF31 and pWEF32 is that pWEF32 has two longer homologous arms. As a result, pWEF32 easily undergoes homologous recombination. On the other hand, pWEF31 undergoes random recombination. The applicability of both vectors was tested by first generating the expression vectors pWEF31-red and pWEF32-red and then detecting the expression of the DsRed2 gene in T. reesei Rut C30. Additionally, we measured the exo-1,4-β-glucanase activity of the recombinant cells. Our work provides an effective transformation system for homologous and heterologous gene expression and gene knockout in T. reesei. It also provides a method for recombination at a specific chromosomal location. Finally, both vectors will be useful for the large-scale gene expression industry.


► Two filamentous fungi Trichoderma reesei expression vectors pWEF31 and pWEF32 were newly constructed by us.
► Both vectors was confirmed by detecting the expression of DsRed2 gene in T. reesei Rut C30.
► The two vectors will be useful for large-scale gene expression industry.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plasmid - Volume 67, Issue 1, January 2012, Pages 67–71
نویسندگان
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