کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
2832863 1163849 2017 11 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
T cell receptor beta chain from sea bream (Sparus aurata): Molecular cloning, expression and modelling of the complexes with MHC class I
ترجمه فارسی عنوان
زنجیره بتا گیرنده سلولT از ماهی دریایی (Sparus aurata): شبیه سازی مولکولی، بیان و مدل سازی از کمپلکس با MHC کلاس I
کلمات کلیدی
T گیرنده آنتی ژن سلول؛ TRB؛ aurata Sparus؛ زمان واقعی PCR؛ ساختار 3D. کلاس I MHC
T cell antigen receptor; TRB; Sparus aurata; Real time PCR; 3D structure; MHC class I
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شناسی مولکولی
چکیده انگلیسی

The T cell receptor is a fundamental mediator of the adaptive immune responses, since TR αβ on T cells recognize foreign structures (peptides derived from processed antigens) bound to the major histocompatibility complex (MHC) on APC cells. In the present study, we report the cloning of six TRB chains cDNA sequences from gilthead sea bream (Sparus aurata), a fish of high economical impact in South Mediterranean aquaculture. The V-BETA domains have the canonical features of known teleost and mammalian TR V-BETA domains and have been divided in four different subgroups. A multiple alignment of the six sea bream TRB chains with other known TRB sequences was assembled and showed the conservation of the four cysteine residues involved in disulphide bonds and of some amino acids with an important role in the assembly and signalling of the TR αβ/CD3 complex. Real-time PCR analysis was used to investigate TRB basal expression, that was maximum in the thymus followed by gut, and TRB in vitro expression after stimulation with LPS or PHA-L at 4 and 24 h (only the 4 h stimulation with LPS gave a significant effect). Moreover, the 3D structures of sea bream TRB chains and MHC-I were predicted by homology modelling with the final aim to investigate the interaction surface in the V-BETA/MHC-I complexes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Immunology - Volume 45, Issue 7, April 2008, Pages 2017–2027
نویسندگان
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