کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
30905 44513 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Quantum dots/particle-based immunofluorescence assay: Synthesis, characterization and application
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Quantum dots/particle-based immunofluorescence assay: Synthesis, characterization and application
چکیده انگلیسی

Recently, it has been proved that quantum dots (QDs) hold the potential to be used in the bioanalysis as fluorescent probes for their many unique optical properties. In this paper, immunofluorescence assay, an integration of particle-based immunoassays and fluorescent QD-probes, was constructed. Firstly, high quality CdSe/ZnS QDs were prepared. Then after being water-solubilized by amphiphilic polymer based on self-assembling, the QDs were labeled by immunoglobulin G (IgG) antibody. At the same time, both carboxyl-polystyrene (PS) and magnetic carboxyl-PS microspheres were prepared and coated by antigens. The antigen sensitized PS microspheres were specifically captured by the QD–IgG bioconjugates based on the antibody-antigen reaction, which was confirmed by the immunofluorescence test in vitro. The sensitivity of current assay was tested by sandwich immunofluorescence assay using human alpha fetoprotein (AFP) as antigen model. The detection limit of AFP antigen is 4.9 ng/mL.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 94, Issue 1, 9 January 2009, Pages 45–50
نویسندگان
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