Förster resonance energy transfer – A spectroscopic nanoruler: Principle and applications

Förster resonance energy transfer (FRET) in association with the recent advancements in optical techniques provides a way to understand the detailed mechanisms in different biological systems at the molecular level. Improvements in wide-field, confocal and two-photon microscopy facilitate the measurements of two-dimensional spatial distribution in steady-state as well as dynamic bimolecular interactions. In the recent decade, FRET became an exceptional fluorescence-based technique due to its potential advantages for studying the biological processes in living cells and more for spatial resolution at nanometer scale. In particular, FRET investigations have shown that biomolecules adopt different conformational structures to perform their functions. In this review, the basic principles and applications of FRET in chemistry, biology, and physics are discussed. Along with, the recent improvements in fluorophore design and labeling and FRET measurement methods are briefly mentioned.

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► This review is about FRET, which includes the basic principle and few recent applications along with, possible measurement techniques.
► Fluorophore labeling methods are described in details as a separate section in this review.
► Application of FRET in research areas ranging from chemistry to biological sciences are described briefly.
► In the final section, further improvements in FRET have been discussed.