کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3299534 | 1209932 | 2007 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reduced Chemokine Receptor 9 on Intraepithelial Lymphocytes in Celiac Disease Suggests Persistent Epithelial Activation
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کلمات کلیدی
mAbCCR9GFDAPCTCrIELMFIPBMCPMA - LDC هاallophycocyanin - آلوفوکسیانینMonoclonal antibody - آنتی بادی مونوکلونالinterleukin - اینترلوکینTransglutaminase - ترانس گلوتامینازGluten-free diet - رژیم غذایی فاقد گلوتنPeripheral blood mononuclear cell - سلول تک هسته ای خون محیطیphorbol myristate acetate - فروبل مریستات استاتIntraepithelial lymphocyte - لنفوسیت داخل اپیتلیالmean fluorescence intensity - میانگین شدت فلورسانسT-cell receptor - گیرنده لنفوسیت T
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
بیماریهای گوارشی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background & Aims: Celiac disease is caused by an inappropriate immune response to dietary gluten, with increased epithelial lymphocyte infiltration in the duodenum/jejunum as a hallmark. The chemokine receptor 9 (CCR9) is a small intestinal homing receptor normally found on most mucosal T cells in this organ. Because CCR9 expression appears to be activation dependent, we examined CCR9 on duodenal T cells from untreated and treated (gluten-free diet) patients with celiac disease and healthy controls. Methods: Duodenal biopsy specimens and blood samples were obtained for histologic analysis and flow-cytometric CCR9 analysis of isolated lymphocytes. CCR9 expression after activation was studied in peripheral blood T cells from healthy volunteers. Results: The median number of CCR9+ cells among CD3+ T cells in epithelium and lamina propria, respectively, was 56% and 48% in controls, 11% and 40% in treated patients, and 1% and 8% in untreated patients. Significant differences occurred between controls and treated or untreated patients in the epithelium but only between controls and untreated patients in the lamina propria (P = .008, all comparisons). No such differences were seen in peripheral blood, but stimulation with phorbol myristate acetate and ionomycin and, to a lesser extent, stimulation via NKG2D reduced the CCR9 expression on blood T cells. Conclusions: CCR9 expression is reduced on epithelial and lamina propria T cells in untreated celiac disease. Down-regulation of CCR9 persists in intraepithelial T cells from well-treated patients. This suggests ongoing immune activation preferentially within the epithelium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gastroenterology - Volume 132, Issue 7, June 2007, Pages 2371-2382
Journal: Gastroenterology - Volume 132, Issue 7, June 2007, Pages 2371-2382
نویسندگان
Richard W. Olaussen, Malin R. Karlsson, Knut E.A. Lundin, Jørgen Jahnsen, Per Brandtzaeg, Inger N. Farstad,