کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
33030 44954 2016 6 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله
Possible practical utility of an enzyme cocktail produced by sludge-degrading microbes for methane and hydrogen production from digested sludge
ترجمه فارسی عنوان
امکان استفاده عملی از کوکتل آنزیمی تولید شده توسط میکروب های تجزیه کننده لجن برای تولید متان و هیدروژن از لجن هضم شده
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Optimal temperature for most DS-hydrolases was ∼50°C.
• DS-hydrolases enhanced CH4 and H2 production from acid-treated DS.
• Methacrylate ester (MAE) can be used as a flocculant for DS.
• DS-hydrolases also enhanced H2 production from MAE-precipitated DS.

Digested sludge (DS) is a major waste product of anaerobic digestion of sewage sludge and is resistant to biodegradation. In this study, we examined suitability of the hydrolases produced by DS-degrading fungal strains (DS-hydrolases) for methane and hydrogen fermentation from DS. Although the strains are mesophilic, DS-hydrolases showed strong chitinase and keratinase activity at ∼50°C. SDS-PAGE analysis suggested that the strains possess a multienzyme system, which allows the hydrolases of some strains to be stable in a wide range of temperatures. Addition of the DS-hydrolases to a vial-scale anaerobic digester enhanced methane and hydrogen production from DS at pH 9.0 and 5.0, respectively. The hydrogen production was also enhanced by the use of methacrylate ester-precipitated DS as a substrate. Further improvement of culture and reaction conditions may make these hydrolases suitable for production of renewable fuels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 33, Issue 1, 25 January 2016, Pages 1–6
نویسندگان
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