کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
33057 44954 2016 8 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation
ترجمه فارسی عنوان
خاموش شدن ژن UDP-glycogen pyrophosphorylases در Phaeodactylum tricornutum بر تخصیص کربن تاثیر می گذارد
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
چکیده انگلیسی


• Suppression of UGPase resulted in significant decreases in chrysolaminarin content.
• Suppression of UGPase led to increases in lipid synthesis.
• UGPase is a rate-limiting enzyme in chrysolaminarin biosynthesis.
• UGPase plays an important role and the allocation of carbon.
• Ugp is a good candidate gene for allocating overall carbon flux in diatoms.

The effects of the suppression of UDP-glucose pyrophosphorylase (UGPase) on chrysolaminaran biosynthesis and carbon allocation were investigated in Phaeodactylum tricornutum. The 69% decrease in UGPase activity was accompanied by a 4.89 fold reduction in Ugp transcript abundance. Inactivation of UGPase in P. tricornutum led to a significant decrease in chrysolaminaran content and an increase in lipid synthesis. These findings suggest that UGPase is a rate-limiting enzyme and may play an important role in chrysolaminarin biosynthesis and carbon allocation. Our results support a theoretical deduction that Ugp is a good candidate for improving lipid synthesis in diatoms.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: New Biotechnology - Volume 33, Issue 1, 25 January 2016, Pages 237–244
نویسندگان
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