کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3349726 1216361 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and characterization of novel HLA alleles: Utility of next-generation sequencing methods
ترجمه فارسی عنوان
شناسایی و مشخص کردن آلل های جدید HLA: سودمند بودن روش های تعیین توالی نسل بعدی
کلمات کلیدی
آلل HLA جدید؛ SBT؛ NGS؛ تعریف Sanger؛ تعیین توالی نسل بعدی؛ آغازگر اختصاصی الل
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی

The HLA genes are the most polymorphic of the human genome, and novel HLA alleles are continuously identified, often by clinical Sanger sequencing-based typing (SBT) assays. Introduction of next-generation sequencing (NGS) technologies for clinical HLA typing may significantly improve this process. Here we compare four cases of novel HLA alleles identified and characterized by both SBT and NGS. The tested NGS system sequenced broader regions of the HLA loci, and identified novel polymorphisms undetected by SBT. Subsequent characterization of the novel alleles in isolation of coencoded alleles by SBT required custom-designed primers, while the NGS system was able to sequence both alleles in phase. However, the tested assay was unable to amplify buccal cell DNA for subsequent NGS sequencing, presumably due to the lower quality of these samples. While NGS assays will undoubtedly increase novel allele identification, more stringent DNA sample requirements may be necessary for this new technology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Human Immunology - Volume 77, Issue 4, April 2016, Pages 313–316
نویسندگان
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