Innate Immune Activation and CD4+ T Cell Priming during Respiratory Fungal Infection

SummaryAspergillus fumigatus is a mold that causes a spectrum of diseases, including lethal lung infections in immunocompromised humans and allergic asthma in atopic individuals. T helper 1 (Th1) CD4+ T cells protect against invasive A. fumigatus infections whereas Th2 CD4+ T cells exacerbate asthma upon inhalation of A. fumigatus spores. Herein, we demonstrate that A. fumigatus-specific T cells were rapidly primed in lymph nodes draining the lung and fully differentiated into interferon-γ (IFN-γ)-producing Th1 CD4+ T cells upon arrival in the airways. T-bet induction in A. fumigatus-specific CD4+ T cells was enhanced by MyD88-mediated signals in draining lymph nodes, but T cell proliferation, trafficking, and Th1 differentiation in the airways were Toll-like receptor (TLR) and MyD88 independent. Our studies demonstrate that CD4+ T cell differentiation during respiratory fungal infection occurs incrementally, with TLR-mediated signals in the lymph node enhancing the potential for IFN-γ production whereas MyD88-independent signals promote Th1 differentiation in the lung.