کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3367991 1218760 2012 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cathepsin S dominates autoantigen processing in human thymic dendritic cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Cathepsin S dominates autoantigen processing in human thymic dendritic cells
چکیده انگلیسی

The interaction of developing thymocytes with peptide-MHC complexes on thymic antigen presenting cells (APC) is crucial for T cell development, both for positive selection of “useful” thymocytes as well as negative selection of autoreactive thymocytes to prevent autoimmunity. The peptides presented on MHC II molecules are generated by lysosomal proteases such as the cathepsins. At the same time, lysosomal proteases will also destroy other potential T cell epitopes from self-antigens. This will lead to a lack of presentation on negatively selecting thymic antigen presenting cells and consequently, escape of autoreactive T cells recognizing these epitopes. In order to understand the processes that govern generation or destruction of self-epitopes in thymic APC, we studied the antigen processing machinery and epitope processing in the human thymus. We find that each type of thymic APC expresses a different signature of lysosomal proteases, providing indirect evidence that positive and negative selection of CD4+ T cells might occur on different sets of peptides, in analogy to what has been proposed for CD8+ T cells. We also find that myeloid dendritic cells (DC) are more efficient in processing autoantigen than plasmacytoid DC. In addition, we observed that cathepsin S plays a central role in processing of the autoantigens myelin basic protein and proinsulin in thymic dendritic cells. Cathepsin S destroyed a number of known T cell epitopes, which would be expected to result in lack of presentation and consequently, escape of autoreactive T cells. Cathepsin S therefore appears to be an important factor that influences selection of autoreactive T cells.


► We study antigen processing in primary human thymic antigen presenting cells (APC).
► Each type of APC has a distinct MHC II-associated processing machinery.
► Plasmacytoid dendritic cells (DC) are slower at processing antigen than myeloid DC.
► CatS dominates processing of myelin basic protein and proinsulin in mDC.
► CatS destroys proinsulin epitopes, limiting deletion of autoreactive T cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Autoimmunity - Volume 38, Issue 4, June 2012, Pages 332–343
نویسندگان
, , , , , , , , , ,