کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
3369646 1219045 2011 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative evaluation of Aptima HIV-1 Qualitative RNA assay performance using plasma and serum specimens from persons with established HIV-1 infection
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
Comparative evaluation of Aptima HIV-1 Qualitative RNA assay performance using plasma and serum specimens from persons with established HIV-1 infection
چکیده انگلیسی

BackgroundBlood specimens for HIV testing are frequently collected using serum collection tubes. The Aptima HIV-1 RNA Qualitative test, originally approved for diagnostic use as a supplemental test for use with plasma, is now approved for use with serum, and may be used in new laboratory-based HIV testing algorithms which detect acute and established HIV infections.ObjectivesTo compare the sensitivity of Aptima using serum and plasma specimens from persons with established HIV infection.Study designParallel serum and plasma specimens were collected from 325 persons with established HIV-1 infection who had positive immunoassay (IA) and Western blot (WB) results. Samples with negative Aptima results were considered false-negative and were subjected to repeat testing. Aptima sensitivity for serum and plasma was calculated relative to IA and WB, and compared using the McNemar test.ResultsThe sensitivity of Aptima using serum (97.23%, 95% confidence interval [CI] 94.81–98.73) was similar to that using plasma (97.54%, 95% [CI] 95.21–98.93) p = 1.00. Five of ten specimens initially false-negative on either serum or plasma were reactive on repeat testing. No specimens initially classified as false-negative on both matrices were reactive on both matrices on repeat testing.ConclusionsIn specimens from persons with established infections, Aptima performed with similar sensitivity when used with serum or plasma. Using serum for immunoassay screening and supplemental testing may provide added convenience for laboratories.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Clinical Virology - Volume 52, Supplement 1, December 2011, Pages S63–S66
نویسندگان
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