کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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3395643 | 1221697 | 2010 | 6 صفحه PDF | دانلود رایگان |
Enzymes in the newly described rumen bacterium, Treponema zioleckii strain kT, capable of digesting Timothy grass fructan, inulin, and sucrose were identified and characterized. Two specific endolevanases and one non-specific β-fructofuranosidase were found in a cell-free extract. The molecular weight of the endolevanases were estimated to be 60 and 36 kDa, whereas that of β-fructofuranosidase, 87 kDa. The former of the specific enzymes was associated with the outer membrane, while the latter and the non-specific β-fructofuranosidase, with the periplasm or cytosol. The Km and Vmax for Timothy grass fructan degradation by endolevanase were 0.27% and 15.75 μM fructose equivalents × mg protein−1 × min−1, those for sucrose and inulin digestion by β-fructofuranosidase were 1.35 × 10−3 M and 1.73 μM hexoses × mg protein−1 × min−1 and 1.77% and 1.83 μM hexoses × mg protein−1 × min−1, respectively.
Journal: Anaerobe - Volume 16, Issue 4, August 2010, Pages 387–392