کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
3420854 | 1594033 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Conformation-sensitive immunoassays improve the serodiagnosis of solitary cysticercus granuloma in Indian patients
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
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چکیده انگلیسی
Neurocysticercosis (NCC), infection of the central nervous system with larva of Taenia solium, presents in over 60% of patients in India as a solitary cysticercus granuloma (SCG). The low cyst number in these patients frequently results in an insignificant humoral response. Consequently, serological tests for patients with SCG must consider the detection of low antibody levels. Lentil lectin-specific T. solium glycoproteins of molecular weights 50, 38, 24, 18, 14 and 13Â kDa are specific antigens for cysticercus antibodies in serological tests for NCC, however they do not detect antibodies in 40% of patients with SCG. To improve this rate of detection, the conformations of these protein antigens were altered to unmask additional epitopes available for antibody binding. Secondary structures of the proteins induced by reduction of disulfide bonds led to the loss of conformational epitopes necessary for cysticercus antibody recognition. Urea-induced tertiary conformations of the antigenic proteins led to the detection of antibodies in 46% of 60 patients with SCG who were serologically negative on immunoblots when the antigens were used in quaternary conformation. Conformation-sensitive immunoassays show potential for serodiagnosis of patients with SCG.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene - Volume 101, Issue 6, June 2007, Pages 570-577
Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene - Volume 101, Issue 6, June 2007, Pages 570-577
نویسندگان
Vasudevan Prabhakaran, Vedantam Rajshekhar, Kenneth Darwin Murrell, Anna Oommen,