کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4328417 | 1614174 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptional regulation of proline-rich membrane anchor (PRiMA) of globular form acetylcholinesterase in neuron: An inductive effect of neuron differentiation
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کلمات کلیدی
ERK3′-5′-cyclic adenosine monophosphate - 3'-5'-cyclic adenosine monophosphatecAMP - cAMPAChE - آهیAcetylcholinesterase - استیل کولین استرازprima - اولNeuron differentiation - تمایز نورونMEK - مجاهدین خلقPromoter regulation - مقررات سازندهmap - نقشهmitogen-activated protein - پروتئین فعال mitogenextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The transcriptional regulation of proline-rich membrane anchor (PRiMA), an anchoring protein of tetrameric globular form of acetylcholinesterase (G4 AChE), was revealed in cultured cortical neurons during differentiation. The level of AChET protein, total enzymatic activity and the amount of G4 AChE were dramatically increased during the neuron differentiation. RT-PCR analyses revealed that the transcript encoding PRiMA was significantly up-regulated in the differentiated neurons. To investigate the transcriptional mechanism on PRiMA regulation, a reporter construct of human PRiMA promoter-tagged luciferase was employed in this study. Upon the neuronal differentiation in cortical neurons, a mitogen-activated protein (MAP) kinase-dependent pathway was stimulated: this signaling cascade was shown to regulate the transcriptional activity of PRiMA. In addition, both PRiMA and AChET transcripts were induced by the over expression of an active mutant of Raf in the cultured neurons. The treatment of a MAP kinase inhibitor (U0126) significantly blocked the expression of PRiMA transcript and promoter-driven luciferase activity as induced by the differentiation of cortical neurons. These results suggested that a MAP kinase signaling pathway served as one of the transcriptional regulators in controlling PRiMA gene expression during the neuronal differentiation process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain Research - Volume 1265, 10 April 2009, Pages 13-23
Journal: Brain Research - Volume 1265, 10 April 2009, Pages 13-23
نویسندگان
Heidi Q. Xie, Roy C.Y. Choi, K. Wing Leung, Vicky P. Chen, Glanice K.Y. Chu, Karl W.K. Tsim,