کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4358742 | 1300454 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lack of correlation between in vitro antibiosis and in vivo protection against enteropathogenic bacteria by probiotic lactobacilli
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
میکروبیولوژی و بیوتکنولوژی کاربردی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Increased resistance to infection is one of the beneficial effects attributed to probiotic microorganisms. This effect may be due to several mechanisms: production of inhibitory substances, blocking of adhesion sites on the intestinal surface, competition for nutrients and stimulation of mucosal and systemic immunity. The present study aimed to investigate the correlation between in vitro and in vivo antimicrobial activity of probiotic lactobacilli. The agar spot test was used to show that twenty Lactobacillus strains were able to inhibit the enteropathogenic bacterium Yersinia enterocolitica. This inhibition was mainly attributable to a decrease in pH resulting from dextrose fermentation by lactobacilli. The inhibition of Y. enterocolitica, Salmonella enterica serovar Typhimurium and Listeria monocytogenes by two probiotic strains, Lactobacillus casei C1 and Lactobacillus plantarum C4, was also associated with the pH decrease. However, both strains lacked protective effects in mouse experimental infection models, with the exception of long-lasting pre-treatment with L. plantarum C4, which exerted a partial protective effect against S. Typhimurium that was attributable to an immunostimulatory mechanism. Our results show that in vitro antibiosis tests do not provide useful information on the probiotic potential of Lactobacillus strains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Research in Microbiology - Volume 165, Issue 1, January 2014, Pages 14-20
Journal: Research in Microbiology - Volume 165, Issue 1, January 2014, Pages 14-20
نویسندگان
Carmen Bujalance, MarÃa Jiménez-Valera, Encarnación Moreno, MarÃa-Dolores Ruiz-López, AgustÃn Lasserrot, Alfonso Ruiz-Bravo,