Mycobiota and mycotoxin contamination of maize flours and popcorn kernels for human consumption commercialized in Spain

Mycobiota and co-occurrence of aflatoxins, citrinin, ochratoxin A and zearalenone in 30 samples of maize flours and 30 of popcorn kernels purchased in Spain for human consumption were determined. The mycotoxin-producing ability of Aspergillus, Fusarium and Penicillium spp. was also studied.Total fungal counts of maize flours ranged from <10 to 8.4 × 104 CFU/g and predominant mycobiota belonged to Aspergillus spp. and Penicillium spp. In popcorn kernels samples the most frequent species were Aspergillus spp., Mucorales, Fusarium spp. and Penicillium spp.Aflatoxins were produced by Aspergillus flavus and Aspergillus parasiticus, citrinin by Penicillium citrinum and Penicillium verrucosum, ochratoxin A by Aspergillus niger and patulin by Aspergillus clavatus and Penicillium griseofulvum. Identification of all the mycotoxin-producing strains as well as some Aspergillus spp. difficult to identify using phenotypic characters only was also performed by molecular methods.Aflatoxins were detected in 14 maize flours and 2 popcorn kernels samples, while ochratoxin A was detected in 4 maize flours and 10 popcorn samples. Co-occurrence of aflatoxins and ochratoxin A was found in the 4 ochratoxin-positive maize flour samples. Citrinin and zearalenone were not detected. This is the first report of aflatoxins and ochratoxin A contamination in maize flours and popcorn kernels commercialized in Spain.

► First report of AFs and OTA in maize flours and popcorn commercialized in Spain.
► Aflatoxins were detected in 47% of maize flours and 7% of popcorn kernels samples.
► Ochratoxin A was detected in 13% of maize flours and 33% of popcorn kernels samples.
► The only OTA-producing species recovered in the studied substrates was Aspergillus niger.
► Aflatoxins were produced by 31% of Aspergillus flavus strains and all Aspergillus parasiticus strains.