کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4411518 | 1307597 | 2010 | 8 صفحه PDF | دانلود رایگان |
The exposure of Inuit people to polychlorinated biphenyls (PCBs) and chlorinated pesticides has been well characterised but little is known regarding their exposure to dioxin-like compounds, which induce toxic effects through binding to the aryl hydrocarbon receptor (AhR). In order to obtain a global measure of persistent organic pollutants in plasma that interact with this signalling pathway, we used a luciferase reporter gene assay to assess the AhR-mediated transcriptional activity elicited by plasma sample extracts from 874 Inuit adults who were recruited in the course of a prospective epidemiological study conducted in Nunavik (Québec, Canada). Several sociodemographic, anthropometric, dietary and lifestyle variables were considered as possible modulating factors of the AhR-mediated activity in multivariate statistical analyses. The geometric mean AhR-mediated activity expressed as 2,3,7,8-tetachlorodibenzo-p-dioxin equivalents was 8.9 pg g−1 lipids (range: <5–144 pg g−1 lipids). PCB-153 concentration measured by high-resolution gas chromatography–mass spectrometry was moderately correlated to AhR-mediated activity (Pearson’s r = 0.53, p < 0.001). Multiple linear regression analyses revealed that age and omega-3 fatty acids in erythrocyte membranes (an index of marine food consumption) were positively associated with plasma AhR-mediated activity (p < 0.001), whereas a negative association was noted with body fat mass (p = 0.037). These results suggest that AhR-mediated transcriptional activity of Inuit plasma extracts is linked to their organochlorine body burden, most likely that of dioxin-like PCBs, polychlorinated dibenzo-p-dioxins and polychlorodibenzofurans. AhR-mediated transcriptional activity measures may prove useful in investigating possible associations between exposure to AhR agonists and adverse health effects in this indigenous population.
Journal: Chemosphere - Volume 80, Issue 2, June 2010, Pages 75–82