کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4480880 1623066 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparative genomics analyses on EPS biosynthesis genes required for floc formation of Zoogloea resiniphila and other activated sludge bacteria
ترجمه فارسی عنوان
تحلیل ژنومیک مقایسه ای بر روی ژن های بیوسنتز EPS مورد نیاز برای شکل گیری توده resiniphila Zoogloea و سایر باکتری های لجن فعال
کلمات کلیدی
ژنوم؛ Zoogloea؛ لجن فعال؛ شکل گیری توده؛ سنتتاز آسپاراژین
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات فرآیندهای سطح زمین
چکیده انگلیسی


• Analyses of two Zoogloea resiniphila genomes revealed the nitrogen and phosphorus metabolisms.
• A large gene cluster required for floc formation was experimentally identified.
• Two asparagine synthase paralogues within this gene cluster were required for floc formation.
• Similar gene cluster(s) were found in other crucial activated sludge bacteria.
• The exopolysaccharide biosynthesis genes vary intraspecifically and interspecifically.

Activated sludge (AS) process has been widely utilized for municipal sewage and industrial wastewater treatment. Zoolgoea and its related floc-forming bacteria are required for formation of AS flocs which is the key to gravitational effluent-and-sludge separation and AS recycling. However, little is known about the genetics, biochemistry and physiology of Zoogloea and its related bacteria. This report deals with the comparative genomic analyses on two Zoogloea resiniphila draft genomes and the closely related proteobacterial species commonly found in AS. In particular, the metabolic processes involved in removal of organic matters, nitrogen and phosphorus were analyzed. Furthermore, it is revealed that a large gene cluster, encoding eight glycosyltransferases and other proteins involved in biosynthesis and export of extracellular polysaccharides (EPS), was required for floc formation. One of the two asparagine synthase paralogues, associated with this EPS biosynthesis gene cluster, was required for floc formation in Zoogloea. Similar EPS biosynthesis gene cluster(s) were identified in the genome of other AS proteobacteria including polyphosphate-accumulating Candidatus Accumulibacter phosphatis (CAP) and nitrifying Nitrosopira and Nitrosomonas bacteria, but the gene composition varies interspecifically and intraspecifically. Our results indicate that floc formation of desired AS bacteria, including CAP strains, facilitate their recruitment into AS and gradual enrichment via repeated AS settling and recycling processes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Water Research - Volume 102, 1 October 2016, Pages 494–504
نویسندگان
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