کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4530405 1324701 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Arsenic inhibits SGK1 activation of CFTR Cl− channels in the gill of killifish, Fundulus heteroclitus
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Arsenic inhibits SGK1 activation of CFTR Cl− channels in the gill of killifish, Fundulus heteroclitus
چکیده انگلیسی

Seawater acclimation in killifish, Fundulus heteroclitus, is mediated in part by a rapid (1 h) translocation of CFTR Cl− channels from an intracellular pool to the plasma membrane in gill and increased CFTR-mediated Cl− secretion. This effect is mediated by serum and glucocorticoid-inducible kinase 1 (SGK1), which is stimulated by plasma hypertonicity rather than cortisol. Since arsenic exposure prevents acclimation to seawater by decreasing CFTR protein levels we tested the hypothesis that arsenic (as sodium arsenite) blocks acclimation to seawater by down regulating SGK1 expression. Freshwater adapted killifish were exposed to arsenic (48 h) and transferred to seawater containing arsenic, and SGK and CFTR expression were measured. Arsenic reduced the seawater induced increase in SGK1 mRNA and protein abundance, and reduced both the total amount of CFTR and the amount of CFTR in the plasma membrane. The decrease in membrane CFTR reduced Cl− secretion. Arsenic also increased the amount of ubiquitinated CFTR and its degradation by the lysosome. Thus, we propose a model whereby arsenic reduces the ability of killifish to acclimate to seawater by blocking the seawater induced increase in SGK1, which results in increased ubiquitination and degradation of CFTR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 98, Issue 2, 10 June 2010, Pages 157–164
نویسندگان
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