کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4550582 1627563 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات اقیانوس شناسی
پیش نمایش صفحه اول مقاله
Crassostrea gigas exposure to the dinoflagellate Prorocentrum lima: Histological and gene expression effects on the digestive gland
چکیده انگلیسی


• Effects of Prorocentrum lima cells were assessed in oyster Crassostrea gigas.
• Prorocentrum lima affected feeding and structural loss of digestive gland.
• Dinoflagellate induced digestive gland modifications in gene expression pattern.
• Physiologic, histopathologic, and genomics analyses useful to estimate bivalve health.

Bivalve mollusks bioaccumulate toxins via ingestion of toxic dinoflagellates. In this study, Crassostrea gigas was used to investigate the effects related to Prorocentrum lima exposure. Oysters were fed with three diets Isochrysis galbana (2 × 106 cell mL−1) control treatment; algal mix of I. galbana (2 × 106) and P. lima (3 × 103 cell mL−1); and P. lima alone (3 × 103 cell mL−1). Feeding behavior changes, histopathological alterations, and expression patterns changes of genes involved in cell cycle (p21, cafp55, p53), cytoskeleton (tub, act), and inflammatory process (casp1) were evaluated. Results indicated that the presence of diarrheic shellfish poisoning by P. lima cells decreased the clearance rate (p < 0.05), induced structural loss, significantly decreased tubule area of the digestive gland (p < 0.05), and up-regulated in expression all gene (p < 0.05), suggesting that toxic cells might trigger inflammatory tissue process, disturb cell cycle and cytoskeleton representing a risk to oysters integrity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Environmental Research - Volume 120, September 2016, Pages 93–102
نویسندگان
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