کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4754267 | 1418056 | 2017 | 12 صفحه PDF | دانلود رایگان |
- To identify the bioactive compounds from Ailanthus excelsa Roxb.
- Phytochemicals analysis was carried out.
- Antioxidant and cytotoxicity activity were screened.
- Bark extract from Ailanthus excelsa exhibited 71% cytotoxicity.
- 55% degradation of sunset yellow dye was observed at 10Â mg/mL of extract.
The purpose of our work is to identify the bioactive compounds of bark and leaves extract from Ailanthus excelsa Roxb. and to explore its effectiveness against synthetic food dye. The presence of primary and secondary metabolites was confirmed by carrying out phytochemicals analysis. With the prior knowledge accessible on the indispensable secondary metabolites holding antioxidant and cytotoxicity activity, the quantitative screening of total phenolic and flavonoid content in methanolic and aqueous extract of bark and leaves from Ailanthus excelsa were done. Comparatively, a higher value of flavonoid (161 ± 0.3 μg/mg) and phenolic acid content (152.4 ± 0.14 μg/mg) was found in bark extract. By FTIR analysis, the characteristic peak was obtained at 1581.63 and 1598.99 cmâ 1 confirmed the presence of functional groups associated to flavonoids and other phenolic groups respectively. In bark extract, 81% of DPPH inhibition was observed when compared to ascorbic acid (standard) 92% of free radical scavenging activity. Bark extract from Ailanthus excelsa exhibited 71% cytotoxicity against HeLa cell line (cervical cancer). In examining the toxicity level of crude extracts with red blood cells (RBC), the bark extract was showed a very less (2.8%) haemolytic activity. They also showed maximum zone of inhibition in antibacterial activity i.e. 13 ± 0.5 mm against Escherichia coli culture. At a concentration of 10 mg/mL of crude extract from A. excelsa, 55% degradation of sunset yellow dye was observed. It concludes that, the compounds present in the A. excelsa, especially the bark extract showed better photocatalytic, haemolytic, antioxidant, cytotoxicity and antibacterial activity when compared to leaves extract.
Graphical Abstract262
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 177, December 2017, Pages 44-55