|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|4765736||1362360||2018||8 صفحه PDF||ندارد||دانلود رایگان|
â¢A class of DCM derivatives with large 2PA cross-section at NIR range were synthesized.â¢PCM was successfully applied for labelling cancer cells with minimal autofluorescence.â¢PCM was successfully utilized to labelling zebrafish with deeper penetration (>300Â Î¼m).
InÂ vitro and inÂ vivo fluorescence imaging of cells and animals plays a significant role in studying biological systems. However, autofluorescence becomes a serious issue inÂ vivo imaging under excitation at UVâvis wavelengths where biological molecules cause interference with the chromophore. In order to address this issue, a novel class of dicyanomethylene-4H-chromene (DCM) derivatives (PCM, PCV, BPCM and BPCV) that can be excited at â¼900Â nm under two-photon excitation conditions and emit in the red wavelength region (â¥600Â nm) have been developed. Particularly, one of the DCM derivatives (PCM) shows several advantages, including remarkable two-photon absorption (2PA) properties in the near-infrared (NIR) region, superior photostability, large Stokes-shift and pronounced solvent-sensitive emission behavior, which is favorable for inÂ vivo fluorescence labelling with a high signal-to-noise ratio.
Graphical abstractA new DCM derivative (PCM) with remarkable two-photon absorption (2PA) properties was successfully applied for labelling cancer cells and zebrafish with minimal autofluorescence and deeper penetration (>300Â Î¼m).Download high-res image (241KB)Download full-size image
Journal: Dyes and Pigments - Volume 148, January 2018, Pages 429-436