کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4765736 1362360 2018 8 صفحه PDF ندارد دانلود رایگان
عنوان انگلیسی مقاله
Rational design of two-photon absorbing dicyanomethylene-4H-chromene derivatives and their application in bioimaging
کلمات کلیدی
DCM derivatives; Large Stokes-shift; Two-photon absorbing; Biological imaging; Minimal autofluorescence;
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Rational design of two-photon absorbing dicyanomethylene-4H-chromene derivatives and their application in bioimaging
چکیده انگلیسی

•A class of DCM derivatives with large 2PA cross-section at NIR range were synthesized.•PCM was successfully applied for labelling cancer cells with minimal autofluorescence.•PCM was successfully utilized to labelling zebrafish with deeper penetration (>300 μm).

In vitro and in vivo fluorescence imaging of cells and animals plays a significant role in studying biological systems. However, autofluorescence becomes a serious issue in vivo imaging under excitation at UV−vis wavelengths where biological molecules cause interference with the chromophore. In order to address this issue, a novel class of dicyanomethylene-4H-chromene (DCM) derivatives (PCM, PCV, BPCM and BPCV) that can be excited at ∼900 nm under two-photon excitation conditions and emit in the red wavelength region (≥600 nm) have been developed. Particularly, one of the DCM derivatives (PCM) shows several advantages, including remarkable two-photon absorption (2PA) properties in the near-infrared (NIR) region, superior photostability, large Stokes-shift and pronounced solvent-sensitive emission behavior, which is favorable for in vivo fluorescence labelling with a high signal-to-noise ratio.

Graphical abstractA new DCM derivative (PCM) with remarkable two-photon absorption (2PA) properties was successfully applied for labelling cancer cells and zebrafish with minimal autofluorescence and deeper penetration (>300 μm).Download high-res image (241KB)Download full-size image

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 148, January 2018, Pages 429-436
نویسندگان
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