کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
4765739 1423887 2018 8 صفحه PDF سفارش دهید دانلود کنید
عنوان انگلیسی مقاله ISI
New series of non-toxic DNA intercalators, mitochondria targeting fluorescent dyes
ترجمه فارسی عنوان
سری جدیدی از intercalators DNA غیر سمی، میتوکندری ها با هدف قرار دادن رنگ های فلورسنت
کلمات کلیدی
رنگ های سیانین منومیتین؛ DNA / RNA؛ ITC؛ دایرهروی دایره ای پارامترهای مرتبط میتوکندریا؛ intercalators
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی


- 5 novel benzoxazolium and benzothiazolium derived dicationic monomethine cyanine dyes.
- Interaction with DNA/RNA: Intercalation with dsDNA; groove binding with dsRNA.
- No antiproliferative activity on cell cultures.
- Fluorescence emission in the mitochondrial space upon cellular uptake.

Five novel benzoxazolium and benzothiazolium derived asymmetric dicationic monomethine cyanine dyes, bearing a thioester group have been synthesised via condensation of quaternary nitrogen containing heterocyclic salts in various anhydrides. Their chemical structures were evaluated by NMR, ESI/MS, IR spectroscopy, and melting point temperatures. Photophysical studies on the title compounds were performed in methanol solutions. The longest wavelength absorption maxima λmax of the studied dyes are in the region 453-508 nm. The corresponding fluorescence maxima λfl were registered within the 489-553 nm. Further spectroscopy and calorimetric techniques including circular dichroism (CD), UV-Vis, fluorescence, thermal denaturation and isothermal titration calorimetry have been applied to the studied dyes in the presence of biopolymers, in order to evaluate their staining ability as fluorescent markers. In aqueous solution the compounds exhibit no intrinsic fluorescence, however upon binding to dsDNA and dsRNA they show considerable enhancement of fluorescence intensity. Binding constants Ks of the complexes [dye-polynucleotide] have been evaluated from fluorescence spectral titrations. Although the intercalation is the predominant binding mode, the compounds show little or no effect on the cell proliferation. Upon fast cell internalization the compounds accumulate in the mitochondrial space.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 148, January 2018, Pages 452-459
نویسندگان
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