Comparison of batch cultivation strategies for cost-effective biomass production of Micractinium inermum NLP-F014 using a blended wastewater medium

- A valuable strategy for cost-effective production of M. inermum was determined.
- Two batch cultivation strategies, fed-batch and sequencing-batch mode, were tested.
- Culture medium was a mixture of domestic WW and aerobically digested swine WW.
- The highest biomass productivity was obtained from the sequencing-batch mode.
- The sequencing-batch mode also showed low environmental impacts.

Two competitive strategies, fed-batch and sequencing-batch cultivation, were compared in cost-effective biomass production of a high lipid microalgae, Micractinium inermum NLP-F014 using a blended wastewater medium. For fed-batch cultivations, additional nutrient was supplemented at day 2 (FB1) or consecutively added at day 2 and 4 (FB2). Through inoculum size test, 1.0 g-DCW L−1 was selected for the sequencing-batch cultivation (SB) where about 65% of culture was replaced with fresh medium every 2 days. Both fed-batch cultivations showed the maximum biomass productivity of 0.95 g-DCW L−1 d−1, while average biomass productivity in SB was slightly higher as 0.96 ± 0.08 g-DCW L−1 d−1. Furthermore, remained concentrations of organics (426 mg-COD L−1), total nitrogen (15.4 mg-N L−1) and phosphorus (0.6 mg-P L−1) in SB were much lower than those of fed-batch conditions. The results suggested that SB could be a promising strategy to cultivate M. inermum NLP-F014 with the blended wastewater medium.