کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5009064 | 1462039 | 2017 | 8 صفحه PDF | دانلود رایگان |
- Preparation of a novel and highly stable GR-CMF composite
- Electrochemical behavior of Hb was studied at Hb immobilized GR-CMF composite
- The high ks of 6.17 sâ1 was achieved for Hb immobilized GR-CMF composite
- A lower LOD (10Â nM) was obtained for H2O2 using Hb immobilized GR-CMF composite
In the present work, we have investigated the electrochemical behavior and electrocatalysis of hemoglobin (Hb) immobilized on a glassy carbon electrode (GCE) modified with a graphene-cellulose microfiber (GR-CMF) composite. The GR-CMF composite was characterized by scanning electron microscopy, elemental analysis, and Raman and Fourier transform infrared spectroscopy. Well-defined electrochemical redox characteristics of Hb were observed for Hb immobilized on a GR-CMF composite modified GCE, with a formal potential of â0.306 V and a peak to peak separation of approximately 67 mV. Due to the high biocompatibility of the GR-CMF composite, the electrochemical behavior of the Hb heme redox couple (FeII/FeIII) was enhanced for Hb immobilized on the GR-CMF composite when compared to Hb immobilized on pristine GR. The heterogeneous electron transfer constant (ks) was calculated as 6.17 sâ1, and is higher than previously reported for Hb immobilized GR supports. The Hb immobilized GR-CMF composite modified electrode was used for the quantification of H2O2 under optimal conditions, and shows a wider linear amperometric response ranging from 0.05 to 926 μM. The limit of detection of the biosensor was 0.01 μM with the sensitivity of 0.49 μA μMâ1 cmâ2. The biosensor also showed high selectivity in the presence of the range of interfering compounds and exhibits good operational stability and practicality in the detection of H2O2.
Journal: Sensors and Actuators B: Chemical - Volume 252, November 2017, Pages 175-182