کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031237 | 1470942 | 2017 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A low cost and palm-size analyzer for rapid and sensitive protein detection by AC electrokinetics capacitive sensing
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کلمات کلیدی
PBSI2COADCMycobacterium avium subsp. paratuberculosisSCLI-VEDLDEPMCUPOCSDAARMacet - ACETBSA - BSADFT - DFTDe-ionized water - آب یونیزه شدهbovine serum albumin - آلبومین سرم گاوUniversal serial bus - اتوبوس سریال جهانیSAW - ارهLCD - ال سی دیInterdigitated electrode - الکترود بین دیجیتالAC electrokinetics - الکتریکینیت ACIDE - اینجاJohne's disease - بیماری جونDiscrete Fourier transform - تبدیل فوریه گسستهEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاAlternating current - جریان متناوبDC, Direct current - جریان مستقیم، جریان پیوستهLOD یا Limit of detection - حد تشخیصElectrical Double Layer - دو لایه برقDI water - دی آبDielectrophoresis - دی الکتر فوروزProtein sensor - سنسور پروتئینliquid crystal display - صفحه نمایش کریستال مایعlimit of detection - محدودیت تشخیصPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریSurface acoustic wave - موج صوتی سطحmap - نقشهpoint of care - نقطه مراقبتUSB - یو اس بی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Specific detection of protein biomarkers has a wide range of applications in areas such as medical science, diagnostics, and pharmacology. Quantitative detection of protein biomarkers in biological media, such as serum, is critically important in detecting disease or physiological malfunction, or tracking disease progression. Among various detection methods, electrical detection is particularly well suited for point-of-care (POC) specific protein detection, being of low cost, light weight and small form factor. A portable system for sensitive and quantitative detection of protein biomarkers will be highly valuable in controlling and preventing diseases outbreaks. Recently, an alternating current electrokinetic (ACEK) capacitive sensing method has been reported to demonstrate very promising performance on rapid and sensitive detection of specific protein from serum. In this work, a low cost and portable analyzer with good accuracy is developed to use with ACEK capacitive sensing to produce a true POC technology. The development of a board-level capacitance readout system is presented, as well as the adaption of the protocol for use with ACEK capacitive sensing. Results showed that the developed system could achieve a limit of detection of 10Â ng/mL, comparable to a sophisticated benchtop instrument. With its small size and light-weight similar to a smart phone, the developed system is ready to be applicable to POC diagnostics. Further, the readout system can be readily expanded for multichannel monitoring and telecommunication capabilities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 83-90
Journal: Biosensors and Bioelectronics - Volume 90, 15 April 2017, Pages 83-90
نویسندگان
Xiaozhu Liu, Cheng Cheng, Jayne Wu, Shigetoshi Eda, Yongcai Guo,