کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5031310 | 1470933 | 2018 | 7 صفحه PDF | دانلود رایگان |
- A capillary multiplexed immunoassay chip comprising an array of tailored pumps was developed.
- The different capillary pumps were designed to obtain different flow rates at each assay step.
- The chip was used to detect 3 relevant mycotoxins (OTA, AFB1 and DON) as proof-of-concept.
- The generated colorimetric signal was acquired using a common smartphone camera.
- All mycotoxins were detected within the relevant limits imposed for feed-stuffs.
The field of microfluidics holds great promise for the development of simple and portable lab-on-a-chip systems. The use of capillarity as a means of fluidic manipulation in lab-on-a-chip systems can potentially reduce the complexity of the instrumentation and allow the development of user-friendly devices for point-of-need analyses. In this work, a PDMS microchannel-based, colorimetric, autonomous capillary chip provides a multiplexed and semi-quantitative immunodetection assay. Results are acquired using a standard smartphone camera and analyzed with a simple gray scale quantification procedure. The performance of this device was tested for the simultaneous detection of the mycotoxins ochratoxin A (OTA), aflatoxin B1 (AFB1) and deoxynivalenol (DON) which are strictly regulated food contaminants with severe detrimental effects on human and animal health. The multiplexed assay was performed approximately within 10Â min and the achieved sensitivities of<40, 0.1-0.2 and<10Â ng/mL for OTA, AFB1 and DON, respectively, fall within the majority of currently enforced regulatory and/or recommended limits. Furthermore, to assess the potential of the device to analyze real samples, the immunoassay was successfully validated for these 3 mycotoxins in a corn-based feed sample after a simple sample preparation procedure.
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Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 40-46