کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031312 1470933 2018 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Streptavidin-hydrogel prepared by sortase A-assisted click chemistry for enzyme immobilization on an electrode
ترجمه فارسی عنوان
استرپتاویدین-هیدروژل تهیه شده توسط checklist کمک گرفته شده توسط مرتب سازی A با استفاده از آنزیم بی حرکت شدن بر روی یک الکترود
کلمات کلیدی
مرتب سازی؛ A؛ استرپتاویدین؛ هیدروژل؛ Bioanode
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- A hydrogel composed of streptavidin and branched PEG was newly prepared.
- Streptavidin was used as a bridge site in the hydrogel backbone.
- The hydrogel was used for immobilizing glucose dehydrogenase on an electrode.
- The bioanode and Pt-carbon cathode were assembled into a glucose/O2 fuel cell.

A tetrameric streptavidin (SA)-appended LPETG tag was site-specifically linked to azido-containing tri-glycine via sortase A catalysis and the resulting azido-modified SA (SA-N3) was retained in the biotin-binding pocket. SA-N3 was polymerized with dibenzylcyclooctyne-modified branched poly(ethyleneglycol) (DBCO-PEG) using azido-modified branched PEG (N3-PEG) as a spacer via copper-free click chemistry. The resulting SA-based hydrogel exhibited gel-like mechanical properties and could immobilize biotin-modified molecules through biotin-SA affinity. Glucose dehydrogenase (GDH) was immobilized in the SA-based hydrogel, and the hydrogel was then coated on a glassy carbon electrode (GCE) and used for the biocatalytic oxidation of glucose. The designed GCE exhibited better performance and stability compared with GDH chemically adsorbed onto a GCE. In addition, the designed GCE anode and a Pt-carbon cathode were assembled into a glucose/O2 fuel cell that provided a maximum power density and open circuit voltage of 11.8 ±0.56 µW cm-2 and 0.17 V, respectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 56-61
نویسندگان
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