کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031317 1470933 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Graphene oxide@gold nanorods-based multiple-assisted electrochemiluminescence signal amplification strategy for sensitive detection of prostate specific antigen
ترجمه فارسی عنوان
استراتژی تقویت سیگنال الکتروکرومیلومینسانس چندگانه مبتنی بر نانوساختار گرافن برای تشخیص حساسیتی از آنتیژن خاص پروستات
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- The GO@AuNRs-GOD-SA as the signal-amplifying probe was prepared for the first time.
- GO@AuNRs-GOD has better electrocatalytic activity to decompose H2O2 to increase luminol ECL.
- The strong interaction of SA with biotin further amplified the ECL intensity.
- The proposed method achieved the highly sensitive detection of PSA.

A novel and competitive electrochemiluminescence (ECL) aptasensor for prostate specific antigen (PSA) assay was constructed using gold nanorods functionalized graphene oxide (GO@AuNRs) multilabeled with glucose oxidase (GOD) and streptavidin (SA) toward luminol-based ECL system. A strong initial ECL signal was achieved by electrodeposited gold (DpAu) on the electrode because of gold nanoparticles (AuNPs) motivating the luminol ECL signal. The signal probes prepared by loading GOD and SA-biotin-DNA on GO@AuNRs were used for achieving multiple signal amplification. In the absence of PSA, the signal probes can be attached on the electrode by hybridization reaction between PSA aptamer and biotin-DNA. In this state, the GOD loaded on the probe could catalyze glucose to in situ produce H2O2 and then AuNRs catalyze H2O2 to generate abundant reactive oxygen species (ROSs) in luminol ECL reaction. Both the high-content GOD and AuNRs in the signal probe amplified the ECL signal in the ECL system. Moreover, the combination of SA with biotin-DNA further expands ECL intensity. The integration of such amplifying effects in this protocol endows the aptasensor with high sensitivity and good selectivity for PSA detection. This aptasensor exhibits a linear relation in the range of 0.5 pg mL−1 to 5.0 ng mL−1 with the detection limit of 0.17 pg mL−1 (S/N = 3). Besides, the strategy was successfully applied in determination of human serum samples with recovery of 81.4-116.0%.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 92-98
نویسندگان
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