کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031346 1470933 2018 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An ultrasensitive micropillar-based quartz crystal microbalance device for real-time measurement of protein immobilization and protein-protein interaction
ترجمه فارسی عنوان
دستگاه میکروبیال بلوری کوارتز مبتنی بر میکروپیلر برای اندازه گیری زمان واقعی از بی حرکت سازی پروتئین و تعامل پروتئین و پروتئین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- A novelsensing device based on resonance between micropillar and QCM substrate (QCM-P) was developed to achieve ultrahigh sensitivity for protein-protein interaction.
- A two-degree-of-freedom theoretical model was established for QCM-P and verified by experimental results.
- The equilibrium binding constant of protein interaction obtained by QCM-P was in good agreement with that measured by SPR.

A new sensing device was developed to achieve ultrahigh sensitivity, by coupling polymer micropillars with a quartz crystal microbalance (QCM) substrate to form a two-degree- of-freedom resonance system (QCM-P). The sensitivity of these QCM-P devices was evaluated by measuring mass changes for both deposited gold film and adsorption of bovine serum albumin (BSA), respectively, on poly(methyl methacrylate) (PMMA) micropillar surfaces, as well as assessing ligand-analyte binding interactions between anti-human immunoglobulin G (anti-hIgG) and human immunoglobulin G (hIgG). The anti-hIgG and hIgG binding results show QCM-P achieved an eightfold improvement in sensitivity relative to conventional QCM sensors. In addition, the binding affinity obtained from the QCM-P device for anti-hIgG and hIgG proteins was found in good agreement with that measured by surface plasmon resonance (SPR) for the same binding reaction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 325-331
نویسندگان
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