کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
5031373 1470933 2018 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chitosan-nickel film based interferometric optical fiber sensor for label-free detection of histidine tagged proteins
ترجمه فارسی عنوان
فیبر کیتوزان-نیکل بر پایه فیبر نوری فتوولتائیک برای تشخیص بدون برچسب از پروتئین های حاوی هیستیدین نشان داده شده است
کلمات کلیدی
تداخل سنجی فیبر نوری، اینترمدال، برچسب پلی هیستیدین، کیتوزان، یون نیکل، میکروکن تریپسین،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


- Chitosan-nickel film based interferometric sensor for histidine tagged proteins.
- Protein of interest hexa-histidine tagged microcin, a bacteriocin.
- Microcin detection sensitivity-0.0308 nm/(ng/ml), detection limit-0.8368 ng/ml.
- The sensor exhibits a quick response of around 150 s.

An interferometric fiber sensor for detection of hexa-histidine tagged microcin (His-MccS) is reported and experimentally demonstrated. This intermodal fiber sensor is implemented by a no-core fiber (NCF) functionalized with chitosan (CS)-nickel (Ni) film for direct detection of small peptide: microcin. The fiber intermodal sensor relies on the refractive index modulations due to selective adsorption event at the chitosan (CS)-nickel (Ni) film. Owing to the strong affinity between Ni2+ ions and histidine, the immobilized Ni2+ ions in the chitosan film were utilized as binding agents for the direct detection of hexa-histidine tagged microcin. A comparative study in relation to different target size was conducted: full proteins trypsin, bovine serum albumin (BSA) and human serum albumin (HSA), with high histidine content on their surface and His-MccS (peptide, 11.6 kDa), have been employed for sensor evaluation. Results have shown selectivity for His-MccS relative to trypsin, BSA and HSA. The most telling contribution of this study is the fast detection of small biomolecule His-MccS compared to standard detection procedures like SDS-PAGE and western blot. The proposed sensor exhibits His-MccS detection sensitivity of 0.0308 nm/(ng/ml) in the range of (0-78) ng/ml with concentration detection limit of 0.8368 ng/ml.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biosensors and Bioelectronics - Volume 99, 15 January 2018, Pages 578-585
نویسندگان
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