ReviewAdvances of Vibrational Circular Dichroism (VCD) in bioanalytical chemistry. A review

- Vibrational Circular Dichroism (VCD) determines absolute configuration of chiral molecules.
- VCD is capable of probing supramolecular chirality of macromolecules, such as protein aggregates and DNA.
- Using VCD, mechanisms of molecule-molecule interactions and chiral separations can be investigated.
- Recent advances of VCD in bioanalytical chemistry are critically discussed.

Vibrational Circular Dichroism (VCD) is a unique and relatively new spectroscopic technique that is capable of determining an absolute configuration of chiral molecules. VCD can be also used to determine structure of large macromolecules. This review highlights the most recent advances of VCD in bioanalytical chemistry. It shows that VCD is capable of unraveling supramolecular organization of peptides, proteins, saccharides, glycerophospholipids, polypeptide microcrystals, as well as amyloid fibrils and DNA. This review also demonstrates how VCD can be utilized to explore molecule-molecule interactions that determine mechanisms of chiral separations in chromatography. It aims to attract attention of scientists from all different research areas demonstrating the strength and capability of this very powerful spectroscopic technique.

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